Journal: Applied Microbiology and Biotechnology

Article Title: The novel antimicrobial peptide PXL150 in the local treatment of skin and soft tissue infections

doi: 10.1007/s00253-012-4439-8

Figure Lengend Snippet: Antimicrobial effect of PXL150 was evaluated in MMC 99 assay against a panel of Gram-positive and Gram-negative bacterial strains and yeast strains in 0.037 % BHI or 50 % h.i. SWF

Article Snippet: The microbicidal effect of PXL150 was tested against S. aureus (American Type Culture Collection (ATCC) 12600), MRSA (ATCC 33591), Streptococcus pyogenes (ATCC 12344), Propionibacterium acnes (ATCC 6919), Staphylococcus epidermidis (ATCC 12228), E. coli (ATCC 11775), P. aeruginosa (ATCC 15442), Klebsiella pneumoniae (Culture Collection, University of Gothenburg (CCUG) 59413, clinical isolate resistant to penicillins, cephalosporins, aztreonam and carbapenems, with the reference strain ATCC 13883), Acinetobacter baumannii (CCUG 58437, clinical isolate resistant to tobramycin, trimsulfa, ciprofloxacin, cefotaxim, ceftazidim, meropenem, pipera/tazobactam, with the reference strain ATCC 19606) and the yeast strains Candida albicans (ATCC 64549), Candida parapsilosis (ATCC 22019), Candida glabrata (CCUG 35267) and Candida krusei (ATCC 6258) using a minimal microbicidal concentration (MMC) assay.

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Journal: ACS Infectious Diseases

Article Title: Insights into the Spectrum of Activity and Mechanism of Action of MGB-BP-3

doi: 10.1021/acsinfecdis.2c00445

Figure Lengend Snippet: Thermal melt curves of gDNA (salmon) and gDNA:MGB-BP-3 Complex. (A) Exemplar melt curve from one experimental repeat, visually representing the different melt curves of gDNA and the gDNA:MGB-BP-3 Complex. Data has been fitted with a Boltzmann distribution. (B) Melting temperatures of gDNA and gDNA:MGB-BP-3 Complex calculated from fitted Boltzmann distributions using OriginPro 2021. All values are an average for n = 4 experimental repeats with an error of ±1 °C.

Article Snippet: Bacterial genomic DNA (ATCC 43300, DSM 13661; ATCC 27853, DSM1117; ATCC 700603, DSM 26371; ATCC 25922, DSM 1103; ATCC 19606, DSM 30007; ATCC 51299, DSM 12956; Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) or salmon genomic DNA (deoxyribonucleic acid sodium salt from salmon testes, D1626, Merck) dissolved in 1 mM phosphate buffer pH 7.4 (containing 0.27 mM potassium chloride, 13.7 mM sodium chloride) to a concentration of 100 μg/mL in SybrSafe (SYBR Safe DNA Gel Stain, ×10,000 in DMSO, S33102 Invitrogen) was used as supplied by the manufacturer in DMSO, and MGB-BP-3 was prepared as 10 mM stock in DMSO.

Techniques:

Journal: ACS Infectious Diseases

Article Title: Insights into the Spectrum of Activity and Mechanism of Action of MGB-BP-3

doi: 10.1021/acsinfecdis.2c00445

Figure Lengend Snippet: Remaining Fluorescence (%) of SybrSafe Probe upon Addition of MGB-BP-3 to gDNA a

Article Snippet: Bacterial genomic DNA (ATCC 43300, DSM 13661; ATCC 27853, DSM1117; ATCC 700603, DSM 26371; ATCC 25922, DSM 1103; ATCC 19606, DSM 30007; ATCC 51299, DSM 12956; Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) or salmon genomic DNA (deoxyribonucleic acid sodium salt from salmon testes, D1626, Merck) dissolved in 1 mM phosphate buffer pH 7.4 (containing 0.27 mM potassium chloride, 13.7 mM sodium chloride) to a concentration of 100 μg/mL in SybrSafe (SYBR Safe DNA Gel Stain, ×10,000 in DMSO, S33102 Invitrogen) was used as supplied by the manufacturer in DMSO, and MGB-BP-3 was prepared as 10 mM stock in DMSO.

Techniques: Fluorescence